Biomed Chromatogr. 2021 Nov 2:e5272. Epub 2021 Nov 2. PMID: 34727378
Revealing the metabolic mechanism of dandelion extract against A549 cells using UPLC-QTOF MS.
Dandelion extract shows potential anticancer activity and is expected to be a new type of natural anti-cancer drug. However, the effect mechanism of dandelion extract to lung cancer cells is still unclear. Here, untargeted metabolomics approach based on liquid chromatography-mass spectrograph (LC-MS) was used to characterize the metabolic responses of A549 cell to dandelion extract exposure, to provide new clues for the anti-tumor mechanism of dandelion extract from the perspective of metabolomics. A total of 16 differentially expressed and time-related metabolites were identified between dandelion extract exposure and control groups. The perturbed metabolic pathways of A549 cells after dandelion extract exposure mainly include the glycerophospholipid metabolism and purine metabolism. These results concluded that dandelion extract may exert anticancer activity by affecting the malignant proliferation, disturbing the stability of cell membrane structure, reducing the adhesion of tumor cells to extracellular matrix and fibronectin and finally inducing tumor cell death.