Curr Eye Res. 2021 Sep 2. Epub 2021 Sep 2. PMID: 34473602
Protective Action of Hydrogen Sulfide-Releasing Compounds against Oxidative Stress-Induced Cataract Formation in Cultured Bovine Lenses.
PURPOSE: The gaseous signalling molecule, hydrogen sulfide (HS) has antioxidant, anti-inflammatory and anti-apoptotic properties. Since oxidative stress has been implicated in the pathogenesis of cataracts and lenticular hydrogen peroxide (HO) is elevated in some cataract patients, the present study investigated the ability of HS-releasing compounds to prevent HO-induced cataract formation in cultured bovine lenses.METHODS: Lenses were cultured in either Dulbelcco's Modified Eagle Medium (DMEM; control); HO(50 mM); ascorbic acid (AA; 3 mM) (positive control); and the HS-releasing compounds (diallyl trisulfide [DATS] or GYY4137) in the presence of HO(50 mM). Lens opacity was determined using a plate reader to measure transmittance. Lens glutathione content (GSH), superoxide dismutase (SOD) activity and lactate dehydrogenase (LDH) cytotoxicity were assessed before and after treatment with the HS-releasing compounds.RESULTS: Both DATS (10M – 10M) and GYY4137 (10M – 10M) significantly (p